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通过基因枪导入法 ,将外源钾通道基因导入水稻中花 8号、中花 9号、中花 13以及 870 6中表达 ,获得了转基因植株。经 PCR和 Southern检测 ,共获得 4 2个表达了钾通道基因的转基因株系。通过二代的筛选并获得了部分株系的纯合系。转基因植株对钾的吸收累积试验表明 ,与对照相比 ,供试转基因株系的吸钾速率和对钾的累积能力都有明显提高。研究表明钾离子通道在植物体内的表达也能改良植物的钾素营养性状。同时也表明植物营养遗传工程与植物的抗病、抗虫基因工程具有同样的可行性
The transgenic rice plants were obtained by introducing the exogenous potassium channel gene into rice Zhonghua 8, Zhonghua 9, Zhonghua 13 and 870 6 by gene gun introduction. A total of 42 transgenic lines expressing the potassium channel gene were obtained by PCR and Southern blot. Through the second generation of screening and access to some lines of homozygous. Accumulation of potassium uptake by transgenic plants showed that both the potassium uptake rate and the potassium accumulation capacity of the transgenic lines tested were significantly increased compared to the control. Studies have shown that the expression of potassium channels in plants can also improve plant potassium nutrition. It also shows that plant nutrition genetic engineering and plant disease resistance, insect-resistant genetic engineering has the same feasibility