论文部分内容阅读
植物名称:石梓(Gmelina arborea)材材类别:侧枝顶芽。切取5毫米左右的茎尖接种。培养条件:基本培养基均为MS。(一)不定芽分化培养基,每升添加1.0毫克BA;(二)幼苗培养基,每升添加0.8毫克BA和0.5～1.0毫克IAA;(三) 生根培养基,每升添加0.5毫克IBA,或先经125ppm浓度的IBA溶液处理,再培养在无激素的液体MS培养基上。培养温度在24℃以上,室内自然散射光。生长和分化情况:(一)在MS+BA 1.0毫克/升培养基上,培养十天以后,逐渐形成3～4个或更多个不定芽,间或有少量幼苗形成,二十天以后如不转移到MS+BA
Botanical Name: Gmelina arborea Type of material: Lateral shoots. Cut about 5 mm of the shoot tip inoculation. Culture conditions: the basic medium are MS. (A) adventitious bud differentiation medium, adding 1.0 mg BA per liter; (b) seedling medium, adding 0.8 mg BA and 0.5-1.0 mg IAA per liter; (c) Rooting medium, adding 0.5 mg IBA per liter, Or first treated with 125ppm IBA solution and cultured on hormone-free liquid MS medium. Culture temperature above 24 ℃, indoor natural light scattering. Growth and differentiation: (a) in the MS + BA 1.0 mg / l medium, after culturing for ten days, gradually formed 3-4 or more adventitious buds, occasionally with a small amount of seedling formation, if not after 20 days Transfer to MS + BA