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本研究用γ射线、EMS诱变,NaCl直接选择10代,获得‘锦橙’、‘桃叶橙’耐盐突变系。耐盐系在无盐胁迫下培养3代,经原生质体分离、培养后再回到盐胁迫下,存活率和生长都正常,这说明耐盐系是稳定的。耐盐系能积累更多的游离脯氨酸,维持较高水平的K~+,吸收较少的Cl~-和Na~+。这很可能是耐盐系耐盐的生理基础。从耐盐系珠心愈伤组织分离原生质体,培养2个月后形成胚状体,胚状体进一步萌发成耐盐原生质体植株。
In this study, γ-ray, EMS mutagenesis, NaCl direct selection of 10 generations, access ’Jin orange’, ’peach orange’ salt-tolerant mutant lines. Salt-tolerant lines were cultivated for 3 generations without salt stress. After protoplast isolation and culture, the salt-tolerant lines were returned to salt stress, and their survival rate and growth were normal, indicating that salt-tolerant lines are stable. Salt tolerant system can accumulate more free proline, maintain a higher level of K ~ +, less absorption of Cl ~ - and Na ~ +. This is likely to be the physiological basis for salt tolerance salt tolerance. Protoplasts were isolated from salt-tolerant calli and cultured for 2 months to form embryoid bodies. Embryoid bodies were further germinated into salt-tolerant protoplasts.