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目的分别检测巨噬细胞炎症蛋白-1α(Mip-1α)敲除小鼠、Mip-1α受体CCR1敲除小鼠和CCR5敲除小鼠腹腔炎性巨噬细胞和粒细胞基质金属蛋白酶-9(MMP-9)的表达,探讨Mip-1α及其受体对于炎性细胞MMP-9表达的影响。方法硫代乙醇酸钠分别诱发野生型小鼠、Mip-1α敲除小鼠、CCR1敲除小鼠和CCR5敲除小鼠无菌型腹腔炎,分别提取腹腔巨噬细胞和粒细胞,鉴定、纯化后,real-timePCR测定MMP-9表达。结果Mip-1α敲除、CCR1敲除、CCR5敲除小鼠的巨噬细胞MMP-9表达低于野生型小鼠(P<0.05),Mip-1α敲除和CCR5敲除小鼠中性粒细胞的MMP-9表达低于野生型小鼠(P<0.05),而CCR1敲除小鼠的中性粒细胞MMP-9表达高于野生型小鼠(P<0.05)。结论Mip-1α敲除和CCR5敲除可降低巨噬细胞和粒细胞MMP-9表达;CCR1敲除可降低巨噬细胞MMP-9表达,升高中性粒细胞MMP-9表达。
Objective To detect the expression of macrophage inflammatory protein-1α (Mip-1α) knockout mice, Mip-1α receptor CCR1 knockout mice and CCR5 knockout mice peritoneal inflammatory macrophages and granulocyte-matrix metalloproteinase-9 (MMP-9), and to explore the effect of Mip-1α and its receptor on the expression of MMP-9 in inflammatory cells. Methods Sodium thioglycollate induced the aseptic peritonitis in wild-type mice, Mip-1α knockout mice, CCR1 knockout mice and CCR5 knockout mice, respectively. Peritoneal macrophages and granulocytes were isolated and identified. After purification, MMP-9 expression was determined by real-time PCR. Results Mip-1α knockout, CCR1 knockout, CCR5 knockout mice macrophages MMP-9 expression was lower than wild-type mice (P <0.05), Mip-1α knockout and CCR5 knockout mice neutrophils The expression of MMP-9 in cells was lower than that in wild-type mice (P <0.05), while the expression of MMP-9 in CCR1 knockout mice was higher than that in wild-type mice (P <0.05). Conclusion Mip-1α knockout and CCR5 knockdown can reduce the expression of MMP-9 in macrophages and granulocytes. CCR1 knockdown can reduce the expression of MMP-9 in macrophages and increase the expression of MMP-9 in neutrophils.