论文部分内容阅读
将小麦白粉菌(Erysiphe graminis f.sp.tritici)的分生孢子接种在含有50ppm 的苯并咪唑琼脂培养基培养的试管幼苗上,放在1～5℃,不加光照的冰箱中保存,通常可以保存150天左右,最高保存时间达180天。随着保存温度的提高,保存时间相应缩短。增加培养基中苯并咪唑的浓度,保存时间略有增加。培养基灭菌与否,与保存时间的长短关系不大,但种子表面消毒处理的比不消毒处理的保存时间显著延长。砂培试管幼苗、土培试管幼苗,在同样条件下,保存时间仅30天左右。在100ppm 苯并咪唑、琼脂培养基中,加入营养液,并不能使保存时间延长。
Conidia of Erysiphe graminis f. Sp. Tritici were inoculated on test tube seedlings grown on benzimidazole agar medium containing 50 ppm and stored in a refrigerator without illumination, usually at 1 to 5 ° C, usually Can save about 150 days, the maximum preservation time of 180 days. As the storage temperature increases, the storage time is reduced accordingly. Increasing the concentration of benzimidazole in the medium increased the storage time slightly. Culture medium sterilization or not, and the duration of the preservation has little to do, but the seed surface disinfection than non-sterile storage time was significantly prolonged. Sand culture tube seedlings, soil culture tube seedlings, under the same conditions, the preservation time of only 30 days. In 100ppm benzimidazole, agar medium, adding nutrient solution, and can not make the storage time lengthen.