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对云南粉棒束孢8个当地居群和蝙蝠蛾拟青霉2个当地居群进行ITS测序和RAPD扩增分析,结合Gen Bank中相关序列,对粉棒束孢开展遗传多样性、居群遗传结构及其种内分化研究。共获得大范围内地理距离远的6个居群共97条粉棒束孢ITS序列,共有33种单倍型,单倍型多样性Hd=0.546和总核苷酸多样性Pi=0.00276,显示粉棒束孢在物种水平上遗传多样性较低。云南粉棒束孢共37条序列,有14种单倍型(10种为云南特有),具有较高单倍型多样性和核苷酸多样性(Hd=0.659,Pi=0.00274);单倍型聚类和网状分支分析表明云南粉棒束孢单倍型类型丰富,遗传多样性高,暗示云南为粉棒束孢多样性分布中心之一。ITS序列分析表明,云南当地居群间遗传分化系数Fst=51.95%;RAPD分析表明,居群间遗传分化系数Gst=0.5547,基因流Nm=0.4014;说明云南当地居群粉棒束孢分化剧烈。居群遗传距离与地理距离相关性研究表明,粉棒束孢居群遗传距离与地理距离无明显相关。中性检验和失配分析表明粉棒束孢经历过近期居群扩张。结合单倍型聚类和网状分支分析,表明Hap 19为扩张建群单倍型,但原始祖先单倍型(Hap 1)依然是粉棒束孢居群中最优势单倍型(频率为48.45%),表明粉棒束孢并不存在明显的因地理原因造成的生殖隔离。值得重视的是,通过ITS单倍型和RAPD分析,支持将蝙蝠蛾拟青霉作为粉棒束孢异名处理。
ITS local sequencing and RAPD analysis were used to analyze the genetic diversity of eight native populations of Myzus persicae and two local populations of Paecilomyces bati in Yunnan Province. Genetic Structure and Intraspecific Differentiation. A total of 97 ITS sequences from 6 populations with a long geographical distance were obtained. A total of 33 haplotypes were found. Haplotype diversity Hd = 0.546 and total nucleotide diversity Pi = 0.00276, indicating that Glaucidium sp. Has a low genetic diversity at the species level. There were 37 sequences in Yunnan, China. There were 14 haplotypes (10 endemic to Yunnan) with high haplotype diversity and nucleotide diversity (Hd = 0.659, Pi = 0.00274) The cluster analysis and reticular branching analysis indicated that the abundance of haploid haplogroups in Yunnan was the highest, with high genetic diversity, suggesting that Yunnan was one of the distribution centers of Dictyostelium discoideum diversity. ITS sequence analysis showed that the genetic differentiation coefficient (Fst) of native populations in Yunnan Province was 51.95%. RAPD analysis showed that the genetic differentiation coefficient among populations was Gst = 0.5547 and gene flow Nm was 0.4014. The correlation between population genetic distance and geographic distance showed that there was no significant correlation between the genetic distance and geographical distance of the population. Neutrality test and mismatch analysis showed that the powder bundle cephalosporins experienced near-term population expansion. Combined with haploid clustering and reticular branching analysis, Hap 19 was shown to be an expanded haplogroup. However, the haplogroup Hap 1 was still the most predominant haplotype (frequency was 48.45%), indicating that there is no obvious genetic isolation caused by geographical reasons. It is important to note that Paecilomyces batmai is supported as a synonym for C. fasciatus by ITS haplotypes and RAPD analysis.