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取健康成年杂交犬34只,不拘雌雄,体重11～21 kg。5％硫苯妥钠静脉麻醉。左肩胛下去毛备皮约6 cm~2。自颈静脉和股静脉各插入一根7F 2极和3极导管电极。2极导管插至右室与心内膜接触以备电击后进行临时起搏用。三极导管插至三尖瓣口处外接电生理检查仪器。同步描记希氏束电图、心腔内心电图及肢体导联心电图。34只犬分为50J(焦耳)8只、80J 10只、170J 8只、≥200J 8只四个电击能量组。电击后当天除2只犬进行病理检查外余32只犬缝合伤口饲养。半个月重复上述电生理检查后处死,立即开胸取出心脏肉眼观察电击损伤部位、范围、形态、性质及有无并发症。按常规取材法取下房室结、希氏束及左右束支近端所在部位的心脏组织,常规固定、脱水、包埋,每只连续切片1500张左右,每
Take healthy adult dogs 34, regardless of sex, weight 11 ~ 21 kg. 5% sodium thiophenolate intravenous anesthesia. Left shoulder blade hair down about 6 cm ~ 2. One 7F 2-pole and 3-pole catheter electrodes were inserted from each of the jugular vein and femoral vein. 2-pole catheter inserted into the right ventricle and endocardial contact to prepare for temporary pacing after shock. Three-pole catheter inserted into the tricuspid valve external electrophysiological examination equipment. Simultaneous tracing His bundle, intracardiac electrocardiogram and limb lead electrocardiogram. 34 dogs were divided into 5 J (J) 8, 80J 10, 170J 8, ≥ 200J 8 only four shock energy group. After the electric shock except for 2 dogs, more than 32 dogs were stitched for wound healing. After repeating the above electrophysiological examination for half a month, the rats were sacrificed and the thoracotomy was removed immediately to observe the location, range, shape, nature and complications of electric shock. According to the conventional method to remove the atrioventricular node, His bundle and around the beam branch proximal to the site of the heart tissue, routine fixed, dehydrated, embedded, each of about 1500 consecutive slices, each