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目的对六味地黄丸的2种原型血中移行成分进行含量测定。方法采用RP-HPLC法建立了六味地黄丸体外指纹图谱及大鼠血清色谱指纹图谱,在此基础上通过标准品对照法确认六味地黄丸的原型血中移行成分并对其进行含量测定。结果确认丹皮酚和马钱素为六味地黄丸原型血中移行成分,测定丹皮酚含量为0.93±0.01mg/g,马钱素含量为0.37±0.01mg/g。结论该方法以六味地黄丸入血成分的含量为指标,为更好地控制其内在质量提供了科学依据。
Objective To determine the content of migrating components in blood of two kinds of prototypes of Liuwei Dihuang Pills. Methods The in vitro fingerprints and rat serum chromatographic fingerprints of Liuwei Dihuang Pills were established by RP-HPLC. On the basis of this, the mimetic components of Liuwei Dihuang Pills were identified and determined by standard reference method. The results confirmed that paeonol and loganin were the migraine components of Liuwei Dihuang Pills. The paeonol content was 0.93±0.01 mg/g and the loganin content was 0.37±0.01 mg/g. Conclusion The method is based on the content of blood components in Liuwei Dihuang Pills, which provides a scientific basis for better control of its intrinsic quality.