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采用营养液培养番茄,通过检测不同铜处理下番茄植物螯合肽、根系分泌物、氨基酸、激素含量的变化,研究外源NO(SNP为供体)缓解铜胁迫的生理生化机制。结果表明,与铜处理相比,外施100μmol·L-1SNP能显著提高番茄幼苗根系和叶片植物螯合肽、叶片氨基酸、根系分泌物中糖(果糖、葡萄糖和蔗糖)及根系ABA和叶片IAA、GA3、ZR的含量;显著降低根系氨基酸、根系分泌物中有机酸(草酸、苹果酸、柠檬酸和琥珀酸)及叶片ABA和根系IAA、GA3、ZR的含量。添加NO清除剂牛血红蛋白后,能显著消除NO的缓解效果。表明外源NO可以诱导番茄幼苗根系和叶片抗性物质的分泌,增强根系与叶片组织对过多铜的螯合能力,从而减缓铜胁迫对番茄幼苗的抑制作用。
To study the physiological and biochemical mechanism of exogenous NO (SNP as a donor) to alleviate copper stress by detecting the changes of chelating peptides, root exudates, amino acids and hormones of tomato plants under different copper treatments. The results showed that 100μmol·L-1 SNP could significantly increase the levels of plant chelating peptides, amino acids, root exudates (fructose, glucose and sucrose) and root ABA and leaf IAA , GA3 and ZR, and significantly reduced the contents of ABA and IAA, GA3 and ZR in roots, amino acids, and root exudates (oxalic acid, malic acid, citric acid and succinic acid). Add NO scavenger bovine hemoglobin, can significantly reduce the NO effect of relief. The results showed that exogenous NO could induce the secretion of resistant substances in roots and leaves of tomato seedlings, and increase the chelation ability of root and leaf tissue to excessive copper, thus reducing the inhibitory effect of copper stress on tomato seedlings.