论文部分内容阅读
目的:观察大鼠脑缺血再灌注后脑组织内巢蛋白的表达以及人参皂甙Rb1(Ginsenoside Rb1,GRb1)对神经的保护机制。方法:阻塞大鼠大脑中动脉2h制备脑缺血再灌注模型,大鼠随机分为缺血再灌注组(I/R组)和GRbl给药组(GRbl组)。两组按不同的再灌注时间(3h、12h、1d、2d、3d、5d、10d)分为7个亚组,应用免疫组织化学技术检测脑内巢蛋白的表达。结果:与I/R组相比,GRbl能减轻脑组织的病理改变,上调各时间点巢蛋白阳性细胞数。结论:GRbl对脑缺血再灌注损伤有神经保护作用,其机制与上调缺血再灌注大鼠脑内巢蛋白表达水平有关。
OBJECTIVE: To observe the expression of Nestin in brain tissue and the protective mechanism of Ginsenoside Rb1 (GRb1) in rats after cerebral ischemia-reperfusion. Methods: Cerebral ischemia-reperfusion model was established by occlusion of middle cerebral artery in rats for 2 hours. The rats were randomly divided into ischemia-reperfusion group (I / R group) and GRbl group (GRbl group). The two groups were divided into 7 subgroups according to different reperfusion time (3h, 12h, 1d, 2d, 3d, 5d, 10d). Immunohistochemistry was used to detect Nestin expression. Results: Compared with I / R group, GRbl could reduce the pathological changes of brain tissue and up-regulate the number of nestin positive cells at each time point. CONCLUSION: GRbl has a neuroprotective effect on cerebral ischemia-reperfusion injury and its mechanism is related to up-regulation of nestin expression in the brain of ischemia-reperfusion rats.