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目的探讨淫羊藿苷对人小细胞肺癌细胞株NCI-H446的作用及其机制。方法将对数生长期NCI-H446细胞分成对照组和淫羊藿苷组。对照组细胞常规培养,淫羊藿苷组细胞培养体系中加入淫羊藿苷(8μmol/L)。各组细胞培养48 h后,利用噻唑蓝检测细胞增殖;流式细胞术检测细胞凋亡;实时定量聚合酶链式反应检测细胞Janus激酶2(JAK2)和信号转导子与转录激活子3(STAT3)基因表达;蛋白质印迹法检测细胞中JAK2、STAT3、磷酸化JAK2(p-JAK2)、磷酸化STAT3(p-STAT3)、Bax和BCL-2蛋白的变化。结果与对照组相比,淫羊藿苷组NCI-H446细胞增殖率明显下降(P<0.05),凋亡率明显升高,NCI-H446细胞JAK2 m RNA与STAT3 m RNA无明显差异。蛋白质印迹法检测显示,淫羊藿苷组NCI-H446细胞JAK2、STAT3总蛋白无明显变化(P>0.05);但p-JAK2、p-STAT3和Bax蛋白明显增加,而BCL-2明显减少(P<0.05)。结论淫羊藿苷能抑制NCI-H446细胞增殖,促进NCI-H446细胞凋亡,其作用可能是通过影响JAK2/STAT3信号传导通路实现的。
Objective To investigate the effect of icariin on human small cell lung cancer cell line NCI-H446 and its mechanism. Methods The logarithmic growth phase NCI-H446 cells were divided into control group and icariin group. Control cells were cultured routinely. Icariin (8μmol / L) was added into the icariin cell culture system. The cells were cultured for 48 h, the cell proliferation was detected by thiazolyl blue staining, the apoptosis was detected by flow cytometry, the expression of Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 STAT3) were detected by Western blotting. The protein expressions of JAK2, STAT3, p-JAK2, p-STAT3, Bax and BCL-2 were detected by Western blot. Results Compared with the control group, the proliferation rate of NCI-H446 cells in icariin group was significantly decreased (P <0.05), and the apoptosis rate was significantly increased. There was no significant difference between JAK2 m RNA and STAT3 m RNA in NCI-H446 cells. Western blotting showed that there was no significant change in the total protein of JAK2 and STAT3 in icariin group NCI-H446 cells (P> 0.05); however, the protein expressions of p-JAK2, p-STAT3 and Bax were significantly increased and BCL-2 was significantly decreased P <0.05). Conclusion Icariin can inhibit the proliferation of NCI-H446 cells and promote the apoptosis of NCI-H446 cells, which may be through the influence of JAK2 / STAT3 signal transduction pathway.