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我们已利用抑制消减杂交技术 (suppressionsubtractivehybridization ,SSH) ,构建了结直肠癌转移相关cDNA消减文库 ,为进一步开展结直肠癌转移相关研究提供了大量的信息[1] 。如何高效、便捷、高通量地差异筛选该文库 ,成为目前急需解决的问题。以往多用Northernblot和反向Nort
We have constructed a cDNA subtractive library for colorectal cancer metastasis using suppressionsubtractive hybridization (SSH), which provides a great deal of information for further research on metastasis of colorectal cancer [1]. How to screen this library efficiently, conveniently and with high throughput has become an urgent problem to be solved. In the past with Northernblot and reverse Nort