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目的研究蓄血证大鼠模型的炎症相关指标(IL-1β、IL-6、IL-4、IL-10、TNF-α、IFN-γ、SOD、MDA)及凝血相关指标(PT、TT、6-Keto-PGF1α、TXB2、ET-1、NO、t-PA、PAI-1)的变化,探讨相关指标与蓄血证的相关性。方法采用间隔24 h两次鼠尾注射内毒素脂多糖(LPS)复制蓄血证大鼠模型,观察大鼠体温、全血及血浆黏度变化,并检测相关炎症指标和凝血指标。结果在第二次给予LPS后,多数大鼠体温上升;2 h后大鼠的血液黏度增加最为显著;3 h内,模型组大鼠的PT、TT值先逐渐下降,然后又逐渐上升,其在2 h附近达到最低点。与正常组比较,模型组的2 h组、3 h组的血浆黏度、全血黏度差异有统计学意义(P<0.01);模型组大鼠血清中TNF-α、IL-1、IL-6、IFN-γ值差异无统计学意义(P>0.05),IL-10值、SOD值、MDA值、t-PA含量值、PAI-1值、t-PA/PAI-1比值差异均有统计学意义(P<0.05,P<0.01);模型组大鼠血浆中NO、6-Keto-PGF1α值、TXB2、6-Keto-PGF1α/TXB2差异均有统计学意义(P<0.05,P<0.01)),ET-1值差异无统计学意义(P>0.05)。结论造模后2 h,大鼠血清及血浆中炎症及凝血相关指标的相应改变,表明大鼠呈现出“瘀”“热”互结状态,与中医蓄血证有一致性。
Objective To investigate the expression of IL-1β, IL-6, IL-4, IL-10, TNF-α, IFN-γ, -Keto-PGF1α, TXB2, ET-1, NO, t-PA, PAI-1) Methods The rats were injected with lipopolysaccharide (LPS) for two hours at 24 h intervals to observe the changes of body temperature, whole blood and plasma viscosity, and to detect the related inflammation indexes and coagulation indexes. Results After LPS administration for the second time, the body temperature of most rats increased; the blood viscosity of rats increased most significantly after 2 hours; within 3 hours, the PT and TT values of rats in the model group decreased first and then gradually increased In the vicinity of 2 h to reach the lowest point. Compared with the normal group, the plasma viscosity and the whole blood viscosity in the model group at 2 h and 3 h were significantly different (P <0.01). The level of TNF-α, IL-1 and IL-6 (P> 0.05). The differences of IL-10, SOD, MDA, t-PA, PAI-1 and t-PA / PAI-1 were all statistically significant (P <0.05, P <0.01). There were significant differences in NO, 6-Keto-PGF1α and TXB2, 6-Keto-PGF1α / TXB2 levels in the model group )), ET-1 value was no significant difference (P> 0.05). Conclusions The changes of inflammation and coagulation related indexes in serum and plasma of rats at 2 h after modeling show that rats exhibit “stasis” and “hot” mutual status, which is consistent with that of TCM.