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目的探讨正常人群与胃癌患者的人类组织相容性复合体(MHC)Ⅰ类相关基因A(MICA)各等位基因,特别是等位基因5.1(A5.1)的基因频率和表型频率的差异,评价胃癌患者中幽门螺杆菌(Hp)感染与MICA各等位基因分布的关系。方法选取100名胃癌患者与220名无血缘关系健康个体对照,采用特异引物聚合酶链法,分析MICA基因的多态性分布;以ureC基因扩增产物确定是否有Hp感染。结果胃癌组MICA的A5.1基因的表型频率和等位基因频率均高于正常人群对照组(P<0.05);同时,MICA A5.1的表型频率和等位基因频率低于正常人群对照组(P<0.05)。结论有MICA A5.1等位基因的个体可能有更大患胃癌的风险,而A5等位基因可能是一个保护性因素。
Objective To investigate the frequency and phenotype frequency of alleles (MICA) of human histocompatibility complex (MHC) gene A (MICA), especially allele 5.1 (A5.1) in normal and gastric cancer patients To evaluate the relationship between Helicobacter pylori (Hp) infection and MICA alleles in patients with gastric cancer. Methods A total of 100 gastric cancer patients and 220 unrelated individuals were selected. The genetic polymorphisms of MICA gene were analyzed by polymerase chain reaction (PCR). The ureC gene amplification product was used to determine whether there was Hp infection. Results The phenotype frequency and allele frequency of A5.1 gene in gastric cancer group were higher than those in normal control group (P <0.05). Meanwhile, the phenotype frequency and allele frequency of MICA A5.1 were lower than those in normal population Control group (P <0.05). Conclusion Individuals with the MICA A5.1 allele may have a greater risk of developing gastric cancer, and the A5 allele may be a protective factor.