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酶催化下水的消除或加成代表了一类重要的生化反应,像柠檬酸、苹果酸、磷酸甘油酸等的脱水。加州大学伯克利的Sehultz小组曾在英国的Nature(1989,388,269)上报道产生能催化此类反应的抗体的途径是,产生一个带有能够除去质子的碱基的结合位点。Schultz最近的一个新想法是,产生一种能稳定OH基质子化形式的抗体结合位点,并且能将它转化为更易离去的形式,H_2O。为了验证这个假设,Uno和Schultz合成了带正电荷的半抗原1,这个半抗原模拟了由于活性位点氨基酸或缓冲液使底物质子化的形式——氧鎓离子4。与非催化反应的速度相比,针对半抗原1产生的一种单克
Enzymatic elimination or addition of water represents an important class of biochemical reactions, such as dehydration of citric acid, malic acid, phosphoglycerate, and the like. The Sehultz team at the University of California, Berkeley, reported in Nature, England (1989, 382, 269) that producing an antibody that catalyzes such reactions is to produce a binding site with a base that can remove protons. A recent new idea from Schultz is to create an antibody binding site that stabilizes the OH-based protonated form of the antibody and converts it to a more accessible form, H 2 O. To test this hypothesis, Uno and Schultz synthesized a positively charged hapten1, which mimicked the oxonium ion4 due to protonation of the substrate by amino acids or buffers in the active site. A single gram for hapten 1 compared to the rate of non-catalytic reaction