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目的探讨肝癌和乙型肝炎患者外周血T淋巴细胞和NK细胞水平的表达及其意义。方法采用流式细胞术(FCM)检测47例肝癌、58例乙型肝炎患者和42例健康人外周血T淋巴细胞和NK细胞的百分含量。结果无论是乙型肝炎组还是肝癌患者组,和健康人比较,CD3+和CD4+下降,CD8+升高,NK水平也下降,但肝癌组的下降无统计学差异;HBV DNA低拷贝组和高拷贝组,和HBV DNA(-)组或健康人比较,CD3+和CD4+以及CD4+/CD8+的比值均下降,CD8+升高,NK水平也下降,低拷贝组和高拷贝组之间比较,以及HBV DNA(-)组和健康人比较,各项指标均无统计学差异;在肝癌患者中,HBV DNA(-)组、HBV DNA低拷贝组和高拷贝组,各组之间两两比较,各项指标却均无统计学差异。结论与健康人相比,乙肝病毒感染及肝癌会导致不同程度的导致机体免疫功能紊乱,间接说明乙肝病毒感染在原发性肝癌的发生发展过程中发挥一定作用。
Objective To investigate the expression of T lymphocyte and NK cell in peripheral blood of patients with hepatocellular carcinoma and hepatitis B and its significance. Methods Flow cytometry (FCM) was used to detect the percentage of T lymphocytes and NK cells in 47 cases of HCC, 58 cases of hepatitis B and 42 healthy controls. Results Compared with healthy controls, both CD3 + and CD4 + levels, CD8 + levels and NK levels also decreased, but there was no significant difference in HCC group compared with healthy controls. HBV DNA low-copy group and high-copy group , The ratio of CD3 + to CD4 + and CD4 + / CD8 + decreased, CD8 + increased and NK level also decreased compared with HBV DNA (-) group or healthy people, and the difference between low copy group and high copy group and HBV DNA (- ) Group compared with healthy people, there was no significant difference among all the indexes. Among the patients with liver cancer, HBV DNA (-) group, low HBV DNA copy group and high copy group, No statistical difference. Conclusion Hepatitis B virus infection and liver cancer can lead to different degrees of immune dysfunction compared with healthy people, which indirectly shows that hepatitis B virus infection plays a role in the occurrence and development of primary liver cancer.