A new macrocyclic diamide, 22-membered macrocyclic diamide, named cyclodicaprylamide(2), and five known compounds, bis(2-ethylhexyl) phthalate(1), ethyl 3,4,5-trimethoxybenzoate(3), ethyl 3,4,5-trimethoxycinnamate (4), (+)-syringaresinol(5), loliolide(6), were isolated from the roots of Rauvolfia yunnanensis Tsiang. Their structures were elucidated based on NMR, 2D NMR, and MS spectrum, respectively. They were obtained from it for the first time. A new macrocyclic diamide, 22-membered macrocyclic diamide, named cyclodicaprylamide (2), and five known compounds, bis (2-ethylhexyl) phthalate (1), ethyl 3,4,5- trimethoxybenzoate (3) Their structures were elucidated based on NMR, 2D NMR, and MS spectrum, respectively. They were isolated from the roots of Rauvolfia yunnanensis Tsiang. (5) obtained from it for the first time.