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目的建立生殖支原体Taqman MGB荧光聚合酶链反应(PCR)检测方法,检测宫颈炎患者生殖支原体感染情况,以期发现生殖支原体感染与女性黏液脓性宫颈炎之间的相关性。方法参照国内、外文献建立Taqman MGB荧光PCR检测技术;采集大连市皮肤病医院性病门诊有黏液脓性宫颈炎的女性患者生殖道分泌物标本74例、体检中心对照组标本63例,应用上述检测方法检测标本中的生殖支原体。结果 Taqman探针荧光PCR法敏感性和特异性均较好。黏液脓性宫颈炎患者生殖支原体阳性率为24.3%,对照组未检测出生殖支原体;除不孕以外其他几项调查内容包括尿道炎症状、非婚性接触史、复发性宫颈炎以及治疗史均与生殖支原体感染有关联(P<0.001),其阳性率分别为17.6%、52.9%、42.5%、100%。结论 Taqman MGB荧光PCR法是目前较为适用的生殖支原体检测方法。生殖支原体感染可能是黏液脓性宫颈炎的致病因素;生殖支原体的检测在指导复发性宫颈炎的治疗中有较为重要的意义。
Objective To establish a method for the detection of Mycoplasma genitalium by Taqman MGB fluorescence polymerase chain reaction (PCR) and to detect the mycoplasma genitalium infection in patients with cervicitis, in order to find the correlation between mycoplasma genitalium infection and female mucopurulent cervicitis. Methods According to the domestic and foreign literatures, Taqman MGB fluorescent PCR detection technique was established. Seventy-four specimens of female patients with mucopurulent cervicitis in the STD clinic of Dalian Dermatology Hospital were collected, and 63 samples of the control center were collected. Methods Mycoplasma genitalium in specimens. Results Taqman probe fluorescence PCR method sensitivity and specificity are good. The positive rate of Mycoplasma genitalium in patients with mucoid cervicitis was 24.3%, while the control group did not detect Mycoplasma genitalium; in addition to infertility several other investigations include urethritis symptoms, history of non-marital contact, recurrent cervicitis and treatment history And mycoplasma genitalium infection (P <0.001), the positive rates were 17.6%, 52.9%, 42.5%, 100%. Conclusion Taqman MGB fluorescent PCR method is currently more applicable method for detection of Mycoplasma genitalium. Mycoplasma genitalium infection may be the cause of mucopurulent cervicitis; detection of genital mycoplasma in the treatment of recurrent cervicitis has more important significance.