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用二烷基亚磷酸酯在水体系中可以高选择性地直接N-磷酰化氨基酸、小肽或其混合物,并可直接进行原位正负离子FAB-MS分析;负离子FAB-MS可以分别检测出混合的20种氨基酸或20种二肽或20种三肽的N-磷酰衍生物的全部组分,且图谱清晰,特征性好。磷酰基的引入对正负离子均有数十倍甚至数百倍的增敏效应。表现出各具特色的裂解特征,可以多重确诊。实验表明磷酰基在正负离子FAB-MS中都是一种增敏效果最好的取代基,其增敏效果还与磷酰基上烃基的长短有关。
With dialkyl phosphites in aqueous system can be highly selective direct N-phosphorylation of amino acids, small peptides or mixtures thereof, and can be directly in situ positive and negative FAB-MS analysis; negative ion FAB-MS can be detected separately The mixed 20 amino acids or 20 dipeptide or 20 tripeptide N-phosphoryl derivatives of all components, and the map clear, and good characteristics. Phosphoryl introduction of positive and negative ions are tens or even hundreds of times the sensitization effect. Show characteristics of the lysis characteristics, you can multiple diagnosis. Experiments show that the phosphoryl group in the positive and negative ions FAB-MS is a sensitizing effect of the best substituent, and its sensitization effect is also related to the length of the phosphoryl hydrocarbon group.