目的：探讨肾综合征出血热（ＨＦＲＳ）毒株及病人血清分型方法。方法：快速荧光灶抑制试验（ＲＦＦＩＴ）。结果：６株来自黑线姬鼠和病人的毒株，其免疫血清对Ⅰ型标准株７６－１１８的中和效价较Ⅱ型标准株（ＵＲ）高４～２５６倍；而另６株来自褐家鼠和大白鼠的毒株，包括Ｇｏｕ３和Ｋ２４的免疫血清，对Ⅱ型标准株ＵＲ的中和效价高于Ⅰ型７６－１１８株８～１２８倍；被检的所有毒株和病人血清都能准确地分型。结论：ＲＦＦＩＴ具有快速、简便、特异和准确灵敏的特点，不仅可检测中和抗体，且适用于ＨＦＲＳ病毒血清学分型。 Objective: To investigate the method of serum typing of hemorrhagic fever with renal syndrome (HFRS) and its patients. Method: Fast Focal Inhibition Test (RFFIT). Results: Six strains of Apodemus agrarius and patient were immunized with 4 to 256 times higher neutralizing titers of type Ⅰ strain 76-118 than those of type II strain (UR), while the other 6 strains were from Rattus norvegicus and rat strains, including the Gou3 and K24 immune sera, neutralize the titers of type II strain UR by 8-128-fold higher than those of strains 76-118; all strains and patients tested Serum can accurately type. Conclusion: RFFIT is rapid, simple, specific and sensitive. It not only can detect neutralizing antibodies, but also is suitable for serological typing of HFRS virus.