实验采用庆大霉素对链霉菌702原生质体致死突变标志的紫外诱变筛选模型,以提高其所产抗真菌生物活性物质的产量。实验结果表明,紫外线处理40 s时,致死率达67.15%,突变率高达20.18%,突变株经过摇瓶筛选获得高产菌株42-23-111,产素单位达到949μg/mL,比出发菌株提高了23.2%。 Experiments using gentamicin on Streptomyces 702 protoplast lethal mutation markers UV mutagenesis screening model to increase the production of antifungal bioactive substances produced by it. The experimental results showed that the lethality was 67.15% and the mutation rate was up to 20.18% when treated with UV light for 40 s. The mutant strains were screened by shaking flask to obtain the high yield strain 42-23-111 and the unit of the yield of hormone was 949 μg / mL, 23.2%.