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目的:探讨长非编码RNAPVT1对人膀胱癌细胞UMUC-3增殖及转移相关的生物学行为的影响。方法:通过组织RNA研究,讨论PVT1在膀胱癌组织中的差异;将si-PVT1转入人膀胱癌细胞UMUC-3,并设立对照组;通过q RT-PCR检测si-PVT1的转染效率;MTS试验研究沉默PVT1对细胞增殖的影响;流式细胞技术检测沉默PVT1对细胞凋亡及周期的影响;Western-blot检测肿瘤增殖、凋亡及周期蛋白的影响。结果 :组织中,q RT-PCR证实在膀胱癌组织中,PVT1的表达量较癌旁组织高;q RT-PCR检测si-PVT1,实验组表达量明显降低;细胞增殖实验表明沉默PVT1后细胞增殖变慢;流式细胞技术表明沉默PVT1后细胞凋亡增加,细胞周期阻滞在G0/G1期;沉默PVT1后,细胞的Bax蛋白表达升高,周期蛋白Cyclin D1表达降低,同时EGFR表达降低。结论:PVT1可作为膀胱癌的候选分子标志。沉默长链非编码RNAPVT1可以抑制膀胱癌细胞的增殖、凋亡和细胞周期。
OBJECTIVE: To investigate the effect of long non-coding RNAPVT1 on the biological behavior of human bladder cancer cell UMUC-3 in proliferation and metastasis. Methods: To investigate the difference of PVT1 in bladder cancer by tissue RNA study. Transfect si-PVT1 into human bladder cancer cell UMUC-3 and establish the control group. Detect the transfection efficiency of si-PVT1 by q RT-PCR. The effects of silencing PVT1 on cell proliferation were investigated by MTS assay. The effects of silencing PVT1 on apoptosis and cell cycle were detected by flow cytometry. The effects of PVT1 on cell proliferation, apoptosis and cyclin were detected by Western-blot. Results: q RT-PCR confirmed that the expression of PVT1 in bladder cancer tissues was higher than that in adjacent non-cancerous tissues. Q-RT-PCR detected si-PVT1 in experimental group, and the expression of PVT1 in experimental group was significantly decreased. Flow cytometry showed that after PVT1 silencing, cell apoptosis increased and cell cycle arrest was in G0 / G1 phase. After silenced PVT1 cells, the expression of Bax protein increased and the expression of cyclin D1 decreased while the expression of EGFR decreased . Conclusion: PVT1 can be used as a candidate molecular marker of bladder cancer. Silencing long chain non-coding RNAPVT1 can inhibit the proliferation, apoptosis and cell cycle of bladder cancer cells.