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研究了过量饮酒情况下乙醇对血液中血红蛋白(Hb)的粒度及其分子结构的影响,以浓度分别为200、300、400、500和600mg/dl的乙醇体外作用Hb 3h,采用动态光散射和激光拉曼散射光谱法对作用后的Hb的粒径和拉曼光谱的变化进行检测分析。结果发现,随着乙醇浓度增加,Hb分子粒径会稍减小,氧合Hb占总蛋白比例增加,Hb处于氧合紧密状态而不易释氧;但当乙醇浓度继续增加到600mg/dl时,Hb粒径开始变大,而氧合Hb占总蛋白比例减小。这些结果有助于解释酗酒者组织缺氧的有关机制。
The effect of ethanol on hemoglobin (Hb) particle size and its molecular structure was studied in the case of excessive alcohol consumption. Hb 3h was treated with ethanol at concentrations of 200, 300, 400, 500 and 600 mg / dl, respectively. Laser Raman scattering spectroscopy was used to detect and analyze the changes of particle size and Raman spectrum of Hb. The results showed that with the increase of ethanol concentration, the particle size of Hb decreased slightly, the ratio of oxygenated Hb to total protein increased, and Hb was in the state of oxygenation and not easy to release oxygen. However, when the concentration of ethanol continued to increase to 600mg / dl, The diameter of Hb began to increase, while the proportion of oxygenated Hb in total protein decreased. These results help explain the mechanisms underlying hypoxia in alcoholics.