论文部分内容阅读
目的利用四氯二苯并二恶英(tetrachlorodibenzo-p-dioxin,TCDD)建立稳定高效的昆明小鼠腭裂模型。方法采用不同剂量的TCDD作用于不同妊娠天数的昆明小鼠,观察胎鼠腭裂的发生情况以及药物的毒性影响,确定诱导形成腭裂的最佳条件。结果孕12.5天的昆明小鼠给予一次性灌胃40μg/kg TCDD,可诱导胎鼠腭裂发生率为98.04%。显微镜下观察:13.5天时,实验组及对照组侧腭突位于舌两侧;14.5天时,对照组侧腭突增大并上抬,呈对向生长,头部开始融合;实验组侧腭突增大,未出现上抬以及融合。15.5和16.5天时,对照组侧腭突完全融合,实验组侧腭突上抬至舌上方,但体积较小,未出现融合,形成腭裂。16.5天时,胎鼠的肝、肺无明显组织学结构异常。结论昆明小鼠于孕12.5天一次性灌胃40μg/kg TCDD可建立稳定高效的小鼠腭裂动物模型;TCDD主要通过延迟侧腭突上抬诱导昆明小鼠腭裂发生。
Objective To establish a stable and efficient Kunming mouse cleft palate model by using tetrachlorodibenzo-p-dioxin (TCDD). Methods Different doses of TCDD were applied to Kunming mice with different gestational days to observe the occurrence of cleft palates and the toxic effects of the drugs. The optimal conditions for the formation of cleft palate were determined. Results Kunming mice of 12.5 days pregnant gave a single intragastric administration of 40μg / kg TCDD, the incidence of cleft palate can be induced to 98.04%. Under the microscope, the experimental group and the control group were located on both sides of the tongue at 13.5 days. On the 14th day, the palatal palatal area of the control group was enlarged and lifted up to the opposite direction and the head started to fuse. In the experimental group, Large, no elevation and fusion. At 15.5 and 16.5 days, the control group had complete fusion of the lateral palatal process and the lateral palatal process of the experimental group was elevated above the tongue, but the volume was small and no fusion occurred. Cleft palate was formed. 16.5 days, fetal rat liver, lung no obvious histological abnormalities. Conclusions A stable and efficient cleft palate animal model was established in Kunming mice by intragastric administration of 40 μg / kg TCDD 12.5 days after pregnancy. TCDD induced the cleft palate in Kunming mice by delayed lateral palatal protrusion.