Dental restorative materials with antimicrobial properties can inhibit bacterial colonization, which may result in a reduction of caries at tooth-filling interaction zones. This study aimed to develop antibacterial glass–ionomer cements(GIC) containing a quaternary ammonium monomer(dimethylaminododecyl methacrylate, DMADDM), and to investigate their effect on material performance and antibacterial properties. Different mass fractions(0, 1.1% and 2.2%) of DMADDM were incorporated into the GIC. The flexure strength, surface charge density, surface roughness and fluoride release were tested. A Streptococcus mutans biofilm model was used. Exopolysaccharides(EPS) staining was used to analyze the inhibitory effect of DMADDM on the biofilm matrix. In addition, biofilm metabolic activity, lactic acid metabolism and the expression of glucosyltransferase genes gtf B, gtf C and gtf D were measured. GIC containing 1.1% and 2.2% DMADDM had flexural strengths matching those of the commercial control(P40.1). DMADDM was able to increase the surface charge density but reduced surface roughness(Po0.05). The incorporation of 1.1% and 2.2% DMADDM elevated the release of fluoride by the GIC in the first 2 days(Po0.05). The novel DMADDM-modified GIC significantly reduced biofilm metabolic activity(Po0.05) and decreased lactic acid production(Po0.05). The quantitative polymerase chain reaction(q PCR) results showed that the expression of gtf B, gtf C and gtf D decreased when mass fractions of DMADDM increased(Po0.05). EPS staining showed that both the bacteria and EPS in biofilm decreased in the DMADDM groups. The incorporation of DMADDM could modify the properties of GIC to influence the development of S. mutans biofilms. In this study, we investigated the interface properties of antibacterial materials for the first time. GIC containing DMADDM can improve material performance and antibacterial properties and may contribute to the better management of secondary caries. This study aimed to develop antibacterial glass-ionomer cements (GIC) containing a quaternary ammonium monomer (dimethylaminododecyl methacrylate, DMADDM) , and to investigate their effect on material performance and antibacterial properties. The flexural strength of the DMADDM were incorporated into the GIC. The flexure strength, surface charge density, surface roughness and fluoride release were tested. A Streptococcus mutans biofilm model was used. Exopolysaccharides (EPS) staining was used to analyze the inhibitory effect of DMADDM on the biofilm matrix. In addition, biofilm metabolic activity, lactic acid metabolism and the expression of glucosyltransferase genes gtf B, gtf C and gtf D were measured. GIC containing 1.1% and 2.2% DMADDM had flexural strengths matching those of the commercial contr The incorporation of 1.1% and 2.2% DMADDM elevated the release of fluoride by the GIC in the first 2 days (Po0 .05). The novel DMADDM-modified GIC significantly reduced biofilm metabolic activity (Po.05) and decreased lactic acid production (Po.05). The quantitative polymerase chain reaction (q PCR) showed was the expression of gtf B, gtf C and gtf D decreased when mass fractions of DMADDM increased (Po.05). EPS staining showed that both both bacteria and EPS in biofilm decreased in the DMADDM groups. The incorporation of DMADDM could modify the properties of GIC to influence the development of S In this study, we investigated the interface properties of antibacterial materials for the first time. GIC containing DMADDM can improve material performance and antibacterial properties and may contribute to the better management of secondary caries.