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提出一种新的细胞膜制剂———硅胶载体细胞膜 (carriercellmembrane,CCM) ,即以硅胶为载体 ,用吸附法将活性细胞膜固定在其表面 .用电子显微镜和表面能谱技术 ,对硅胶CCM的表面特性进行了分析 .以K+,Na+ 三磷酸腺苷 (K+,Na+ ATP)酶作为活性指标 ,比较了硅胶CCM与悬液细胞膜和沉淀状细胞膜的酶活性随温度、时间的变化规律和稳定性 .结果表明 ,硅胶CCM与其他两种赋存状态的细胞膜一样 ,具有可比较的酶活性特征 .建立了以硅胶CCM为固定相的色谱系统 ,并用于研究钙拮抗剂与兔红细胞膜和心肌细胞膜特异性相互作用 ,以及二氢吡啶类异构体药物与兔小脑细胞膜立体相互作用 .表明用硅胶CCM色谱法所得色谱参数可以反映药物与膜受体相互作用的特异性和立体选择性 .
A new cell membrane preparation, carriercellmembrane (CCM), was prepared by immobilizing the active cell membrane on its surface with silica gel as support.Using electron microscopy and surface energy spectroscopy, the surface of silica gel CCM (K +, Na + ATPase) as the activity index, the change law and stability of enzyme activity with temperature and time of CCM and suspension cell membrane were compared.The results showed that the activity of K + Silica gel CCM has the same enzymatic activity characteristics as the other two cell membranes, and has established a chromatographic system using silica gel CCM as the stationary phase and is used to study the specific interaction between calcium antagonist and rabbit erythrocyte membrane and myocardial cell membrane , As well as dihydropyridine isomers and rabbit cerebellum membrane three-dimensional interaction.It shows that the chromatographic parameters obtained by silica gel CCM chromatography can reflect the specificity of the drug and membrane receptor interaction and stereoselectivity.