目的探讨急性冠状动脉综合征(acute coronary syndrome,ACS)患者行冠状动脉(冠脉)内支架置入术前、后血小板活化指标的变化。方法利用流式细胞术(flowcytometry,FCM)和单克隆抗体测定45例不稳定型心绞痛(unstable angina pectoris,UAP)与37例急性心肌梗死(acute myocardial in-farction,AMI)患者外周血中血小板膜糖蛋白CD62p,CD63和凝血酶敏感蛋白(thrombin-sensitive protein,TSP)的阳性表达率,并与45例冠脉造影正常者相对照。结果FCM可简单、迅速地检测血小板的活化功能。ACS患者支架置入后CD62p,CD63和TSP的阳性表达率均显著高于支架置入前;ACS组治疗前亦高于对照组。结论急性冠脉综合征伴随血小板活化,介入治疗进一步加强血小板的活化。 Objective To investigate the changes of platelet activation in patients with acute coronary syndrome (ACS) before and after coronary stent implantation. Methods Flow cytometry (FCM) and monoclonal antibody assay were used to detect platelet membrane in peripheral blood of 45 patients with unstable angina pectoris (UAP) and 37 patients with acute myocardial infarction (AMI) The positive rates of glycoprotein CD62p, CD63 and thrombin-sensitive protein (TSP) were compared with those of 45 normal coronary angiography. Results FCM can detect platelet activation function easily and quickly. The positive expression rates of CD62p, CD63 and TSP in ACS patients after stent implantation were significantly higher than those before stent implantation; ACS group before treatment was also higher than the control group. Conclusion Accompanied with platelet activation in acute coronary syndrome, interventional therapy further enhances platelet activation.