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目的:建立微量样品羟自由基(·OH)的检测方法,并用于新疆葡萄树伤流液及金银花茶、罗布麻茶、茉莉茶、竹叶茶等样品的检测。方法:采用Mn2+-H2O2-EBT法,检测波长为560 nm,在2 m L的比色皿中,取缓冲溶液(Na B4O7-Na OH)300μL,EBT 120μL,Mn2+300μL,H2O2150μL,将体系体积用水补充至2.0 m L。Mn2+-H2O2-EBT法可作为检测抗氧化物质抗氧化活性的有效可行方法之一,为验证该方法对微量样品抗羟自由基(·OH)活性实时检测的可行性,以抗坏血酸对照。结果:实验结果表明,新疆葡萄树伤流液、金银花茶、罗布麻茶、茉莉茶和竹叶茶均具有较强的清除羟自由基(·OH)的生物活性功能,是一种很好的天然抗氧化及自由基清除剂。结论:微量样品抗羟自由基检测方法可行,实现吸光度测定的同时,对少量样品进行实时、恒温及震荡在线检测,连续观察吸光度的变化。
OBJECTIVE: To establish a method for the determination of hydroxyl radical (· OH) in trace samples and to detect grapevine injury fluid and honeysuckle tea, apocynum tea, jasmine tea and bamboo leaf tea in Xinjiang. Methods: The detection wavelength was 560 nm with a Mn2 + -H2O2-EBT method. In a 2 mL cuvette, 300 μL of buffer solution (Na B4O7-Na OH), 120 μL of EBT, 300 μL of Mn2 + Make up to 2.0 m L with water. Mn2 + -H2O2-EBT method can be used as an effective and feasible method for detecting the antioxidant activity of antioxidants. To verify the feasibility of this method for real-time detection of · OH activity of trace samples, the ascorbate control was used. Results: The experimental results showed that grapevine injury fluid, honeysuckle tea, apocynum tea, jasmine tea and bamboo leaf tea in Xinjiang have a strong ability of scavenging hydroxyl radical (· OH) biological activity, which is a good Natural antioxidant and free radical scavengers. Conclusion: It is feasible to detect the free-radical scavengers in trace samples. The absorbance measurement is carried out. Meanwhile, a small amount of samples are detected on-line in real time, in constant temperature and in shock. The changes of absorbance are observed continuously.