论文部分内容阅读
目的:研究HIF-1α和Bcl-2在肺腺癌中的表达和相关性,探讨HIF-1α与细胞凋亡的关系。方法:用免疫组化法检测54例肺腺癌和30例癌旁组织中HIF-1α和Bcl-2的表达。结果:HIF-1α在肺腺癌组织中和癌旁组织中表达阳性率分别为33.33%和6.67%,具有显著性差异(P<0.01);Bcl-2在二者中表达阳性率分别为59.26%和16.67%,差异不显著(P>0.05);在肺腺癌组织中,HIF-1α表达随着Bcl-2的升高而降低,二者具有统计学差异(P<0.01),且经过Spearman相关性分析,二者呈明显负相关(r=-0.453,P=0.001)。而HIF-1α在高中分化和低分化肺腺癌中表达率则分别为9.52%和48.48%;在Ⅰ期和Ⅱ、Ⅲ期肺腺癌中表达分别为21.43%和46.15%,组间差异显著(P<0.01),其与年龄、性别则无显著相关(P>0.05)。结论:HIF-1α和Bcl-2在肺腺癌中的表达呈明显负相关。HIF-1α的表达是肿瘤发生的早期事件。
Objective: To investigate the expression of HIF-1α and Bcl-2 in lung adenocarcinoma and to investigate the relationship between HIF-1α and apoptosis. Methods: The expressions of HIF-1α and Bcl-2 in 54 cases of lung adenocarcinoma and 30 cases of paracancerous tissues were detected by immunohistochemistry. Results: The positive rates of HIF-1α expression in lung adenocarcinoma tissues and paracancer tissues were 33.33% and 6.67%, respectively (P <0.01). The positive rates of Bcl-2 expression were 59.26 % And 16.67%, respectively. There was no significant difference between the two groups (P> 0.05). In lung adenocarcinoma, the expression of HIF-1α decreased with the increase of Bcl-2, Spearman correlation analysis showed a significant negative correlation (r = -0.453, P = 0.001). However, the expression rates of HIF-1αin high-grade and poorly differentiated lung adenocarcinomas were 9.52% and 48.48%, respectively. The positive rates of HIF-1α in stage Ⅰ, stage Ⅱ and stage Ⅲ lung adenocarcinomas were 21.43% and 46.15% (P <0.01), but no significant correlation with age and gender (P> 0.05). Conclusion: The expression of HIF-1α and Bcl-2 in lung adenocarcinoma is negatively correlated. HIF-1α expression is an early event in tumorigenesis.