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以野生番茄契斯曼尼LA0317与栽培番茄自交系9706为亲本,构建了包括152个株系的BC2S6群体。利用155个SSR标记、21个CAPS标记和88个Indel标记构建了契斯曼尼番茄的遗传连锁图谱,总图距1134.0cM,标记间平均距离4.29cM。采用复合QTL区间作图法对番茄果实中的可溶性固形物含量和β-胡萝卜素含量进行QTL分析。定位到10个与可溶性固形物含量相关的QTLs,分别位于第3、4、5、6、9、10、11号染色体上,其中有5个QTL效应值在12%以上。定位到6个与β-胡萝卜素含量相关的QTLs,分别位于第1、2、6、9号染色体上。其中,位于6号染色体上的qBC6b效应值达到54.7%,为一主效QTL。此外,还获得了可用作育种材料的5个高可溶性固形物含量株系和8个高β-胡萝卜素含量的株系。
The BC2S6 population including 152 lines was constructed from the parents Chesmaneni LA0317 and cultivated tomato inbred line 9706. The genetic linkage map of Cusmani was constructed using 155 SSR markers, 21 CAPS markers and 88 Indel markers with a total distance of 1134.0 cM and an average distance between markers of 4.29 cM. QTL analysis of soluble solids content and β-carotene content in tomato fruits was performed by composite QTL interval mapping. Ten QTLs related to soluble solids content were mapped on chromosomes 3, 4, 5, 6, 9, 10 and 11, respectively, of which 5 QTLs were above 12%. Six QTLs related to β-carotene were mapped to chromosomes 1, 2, 6 and 9, respectively. Among them, the QBC6b effect on chromosome 6 reached 54.7%, which was a major QTL. In addition, five high soluble solids content lines and eight high beta-carotene content lines were also used as breeding material.