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目的 研究乙肝病毒 (HBVx)和黄曲霉毒素(AFB1)在诱发肝癌中的分子机理。方法 用AtlasTM cDNA表达阵列方法比较研究HBVx基因转基因小鼠HBVx基因整合 (+)和 (- )的小鼠肝组织 ,以及转基因小鼠经AFB1一次攻击的肝癌组织的基因表达谱的差异。结果 经HBVx基因整合 (+)的小鼠肝组织的DNA修复基因 ,药物代谢酶基因以及应激反应的效应因子的cDNA基因表达谱显示了明显的变化。实验结果提示出现变异的 30个cDNA基因表达谱中 2 9个 (96 .7% )与X基因的整合相关及 17个 (5 6 .7% )与AFB1的攻击有关。结论 HBV对宿主的感染 ,可能通过改变DNA修复系统和药物代谢酶系统来影响机体对AFB1的致癌敏感性 ,从而提高AFB1对肝组织的致癌效应
Objective To study the molecular mechanism of hepatitis B virus (HBVx) and aflatoxin (AFB1) in the induction of liver cancer. Methods Atlas TM cDNA expression array was used to compare the gene expression profiles of HBVx gene-integrated (+) and (-) HBVx transgenic mice and the liver cancer tissues of transgenic mice once challenged with AFB1. Results The gene expression profiles of DNA repair genes, drug metabolizing enzyme genes and stress response effectors in liver tissues of HBVx-gene-integrated (+) mice showed obvious changes. The experimental results indicated that 29 (96.7%) of the 30 cDNA gene expression profiles correlated with the integration of X gene and 17 (56.7%) were related to AFB1 attack. Conclusion HBV infects the host and may affect the carcinogenic sensitivity of AFB1 to AFB1 by changing the DNA repair system and drug metabolism enzyme system, thereby enhancing the carcinogenic effect of AFB1 on liver tissue