观察了低能量5-氨基乙酰丙酸介导的光动力学疗法(ALA-PDT)对脑组织新生血管形成和胶质瘤生长的影响。用10J/cm2的ALA-PDT照射27只裸小鼠大脑皮层后第1、5、10天,利用二维、三维图像观察新生血管形成,western blot检测缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)表达。另将12只裸小鼠分为对照组(无特殊处理)和ALA-PDT预处置组(10J/cm2),ALA-PDT预处置后第10天,颅内接种U87胶质瘤细胞,21天检测肿瘤体积。结果发现,以照射区对侧大脑相应区域为对照,低能量ALA-PDT照射后第1和5天,血管形态无明显变化,第10天有新生血管形成;VEGF在照射后第5和10天显著增高;HIF-1α在照射后第1天即开始增高,随后越发明显。低能量ALA-PDT可通过HIF-1α诱导VEGF高表达和新生血管形成,这种微环境的改变有助于U87脑胶质瘤细胞的生长。 The effect of low-energy 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) on neovascularization and glioma growth was observed. The neovascularization was observed by 2D and 3D images on day 1, 5, and 10 of 27 nude mice exposed to 10 J / cm2 ALA-PDT. Western blot was used to detect the expression of hypoxia inducible factor-1α (HIF-1α) And vascular endothelial growth factor (VEGF) expression. Twelve nude mice were divided into control group (without special treatment) and ALA-PDT preconditioning group (10J / cm2). On the 10th day after ALA-PDT preconditioning, U87 glioma cells were intracranially inoculated for 21 days Tumor volume was measured. The results showed that the corresponding regions of the opposite side of the irradiated area were used as controls. There was no significant change of blood vessel morphology on the 1st and 5th days after the low-energy ALA-PDT irradiation. On the 10th day, the neovascularization was formed. On the 5th and 10th day after the irradiation Significantly increased; HIF-1α began to increase on the first day after irradiation, then became more obvious. Low-energy ALA-PDT induces VEGF overexpression and neovascularization via HIF-1α, a change in the microenvironment that contributes to the growth of U87 glioma cells.