杂交瘤技术的一个明显缺点就是依赖对动物的免疫。通过适当的免疫方法去获取大量活化的、抗原特异性的B淋巴细胞,以此作为亲代细胞与骨髓瘤细胞相融合。经典的免疫动物的方法一般需要毫克量的抗原和数周的免疫时程。这种对动物免疫的依赖性及免疫方法上的缺点给杂交瘤技术的广泛应用带来了一定的局限性。首先,开展人—人杂交瘤技术就难以实行对人体进行免疫,然后取其淋巴细胞(如脾脏、淋巴结、外周血)进行融合,其次,制备抗“自身”成份的单克隆抗体时,“自身”抗原在 One of the obvious disadvantages of hybridoma technology is its dependence on animal immunity. A large number of activated, antigen-specific B lymphocytes are obtained by appropriate immunization methods to fuse with myeloma cells as parental cells. Classical methods of immunizing animals generally require milligram quantities of antigen and weeks of immunization. This dependence on animal immunity and the disadvantages of immunological methods have brought some limitations to the widespread use of hybridoma technology. First of all, it is difficult to carry out the human-human hybridoma technique to immunize the human body and then fuse the lymphocytes (such as the spleen, lymph nodes and peripheral blood). Secondly, in the preparation of the monoclonal antibody against “self” "Antigen is here