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目的采用HPLC法测定复方曼陀罗药水中硫酸阿托品的含量方法。方法色谱柱为C18柱,流动相为乙腈-0.4%磷酸(三乙胺调pH5.7)(15:85),检测波长为257 nm。结果线性范围为2~20μg.ml-1(r=0.9999),平均回收率为89.2%,RSD=1.25%。结论新建方法简便、准确、易行。
Objective To determine the content of atropine sulfate in compound mandala potion by HPLC. Methods The column was C18 with mobile phase of acetonitrile-0.4% phosphoric acid (adjusted to pH5.7 with triethylamine) (15:85) at a detection wavelength of 257 nm. The linear range was 2 ~ 20μg.ml-1 (r = 0.9999). The average recovery was 89.2% and RSD was 1.25%. Conclusion The new method is simple, accurate and easy to implement.