[目的]建立南通地区B型HBV流行株PreS基因参考序列。[方法]扩增HBV慢性无症状携带者的PreS基因,分析确定基因型并拟定参照序列。将所测基因序列与HBV不同型PreS序列用Clustalx1.8软件比对,经MEGA3.1分型,选取其中的B型HBV基因,建立南通地区B型HBV流行株的PreS参考序列。[结果]与GenBank收录的HBV-B型序列比较,核苷酸和氨基酸的同源率分别为98.5%～99.2%和96.6%～98.9%。[结论]成功建立了南通地区B型HBV流行株的PreS基因参照序列。 [Objective] To establish the PreS gene reference sequence of B type HBV epidemic strains in Nantong area. [Method] PreS gene of chronic asymptomatic carriers of HBV was amplified, genotypes were determined and reference sequences were drawn. The sequence of the tested gene was compared with that of the different PreS sequences of HBV using Clustalx1.8 software. The B gene of HBV was selected by MEGA3.1 and the PreS reference sequence of the B type HBV epidemic in Nantong was established. [Result] Compared with the HBV-B sequence contained in GenBank, the nucleotide and amino acid identities were 98.5% -99.2% and 96.6% -98.9%, respectively. [Conclusion] The PreS gene reference sequence of B type HBV epidemic strains in Nantong area was successfully established.