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目的为了探索简便、有效的涎腺上皮细胞的体外培养方法。方法采用自制鼠尾胶原胶为底物．在培养液中加入一些促进上皮细胞生长分化的刺激物，对出生一天的Wistar大鼠颌下腺上皮细胞进行原代培养。通过相差显微微镜、光镜及透射电镜对上皮细胞的生长及分化进行观察和研究。结果培养的大鼠颌下腺上皮细胞呈多层生长且为极性分化，保留了腺泡导管分泌单位的三种上皮成份。结论本研究所采用的方法是一种简便、可行、有效的涎腺上皮细胞的体外培养方法，其中自制胶原胶是本研究培养成功的关键。
Objective To explore a simple and effective salivary gland epithelial cells in vitro culture methods. Methods Self-made rat tail collagen glue as a substrate. Some stimulating agents that promote the growth and differentiation of epithelial cells were added to the culture medium to culture primary submandibular gland epithelial cells of Wistar rats on the first day after birth. The growth and differentiation of epithelial cells were observed and studied by phase contrast microscopy, light microscopy and transmission electron microscopy. Results The cultured rat submandibular gland epithelial cells grew in multiple layers and became polarized, retaining the three epithelial components of acinar duct secreting units. Conclusion The method used in this study is a simple, feasible and effective method of in vitro culture of salivary gland epithelial cells. The self-made collagen glue is the key to the success of this study.