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目的 探讨肝细胞癌DNA异倍体、nm2 3 H1、C erbB 2和p5 3癌基因蛋白表达与肝细胞癌侵袭性的关系及其临床意义。方法 采用DNA图像分析系统定量测定 5 2例肝癌患者的肝癌细胞的DNA含量 ,用免疫组化方法检测癌基因蛋白在肝癌中的表达。结果 DNA异倍体在≤ 5cm肝癌组为 5 0 .0 % (12 /2 4) ,在 >5cm肝癌组为 82 .1%(2 3/2 8) ;DNA异倍体与肝癌肝内转移及合并癌栓有关。肝癌伴有肝内转移者的nm2 3 H 1阳性率明显高于不伴肝内转移者。肝癌合并癌栓者的 p5 3蛋白阳性率高于不合并癌栓者。异倍体肝癌的nm 2 3 H1和 p5 3蛋白阳性率高于二倍体肝癌。C erbB 2阳性率在各组中未见明显差别。术后生存率可能与DNA异倍体、nm 2 3 H1和 p5 3蛋白的阳性表达有关。结论 DNA异倍体、nm2 3 H1和 p5 3蛋白阳性表达与肝癌侵袭性有较密切关系 ,检测这些指标有助于根据肝癌的个体差异选择治疗措施 ,提高疗效
Objective To investigate the relationship between the expression of DNA aneuploidy, nm23 H1, C erbB 2 and p53 oncogene proteins and the invasiveness of hepatocellular carcinoma in hepatocellular carcinoma and its clinical significance. Methods DNA image analysis system was used to quantitatively determine the DNA content of hepatocellular carcinoma cells in 52 patients with hepatocellular carcinoma and to detect the expression of oncogene protein in hepatocellular carcinoma by immunohistochemistry. Results DNA aneuploidy was 50.0% (12/24) in ≤ 5 cm HCC and 82.1% (2 3/2 8) in> 5 cm HCC; and the ratio of DNA aneuploidy to liver metastasis in HCC And associated with cancer thrombosis. The positive rate of nm23 H 1 in liver cancer with intrahepatic metastasis was significantly higher than those without liver metastasis. The positive rate of p5 3 protein in patients with hepatocellular carcinoma associated with tumor thrombus was higher than those without tumor thrombus. The positive rates of nm 2 3 H1 and p5 3 protein in aneuploid liver cancer were higher than those in diploid liver cancer. The positive rate of C erbB 2 was not significantly different among the groups. Postoperative survival rate may be related to DNA aneuploidy, nm 2 3 H1 and p5 3 protein expression. Conclusion DNA aneuploidy, nm23 H1 and p5 3 protein expression is closely related to the invasiveness of liver cancer, detection of these indicators help to select treatment based on individual differences in liver cancer, improve the efficacy