论文部分内容阅读
目的研究丹参不同提取物的超高效液相色谱(UPLC)图谱与抗炎作用的谱效关系,为明确丹参抗炎作用物质基础提供依据。方法采用UPLC法对丹参不同提取部位(S1~S9)进行分析,以人支气管上皮细胞炎症模型考察不同提取部位的抗炎作用,采用灰色关联度分析法建立其谱效关系。结果丹参醇提物50%乙醇洗脱液(S3)、80%乙醇洗脱液(S4)、滤过沉淀(S5)以及萃取后水层提取物(S9)对于炎症细胞抗炎能力强,其中以丹参醇提物的50%乙醇洗脱液(S3)作用能力最强。丹参发挥抗炎作用的药效是多种成分共同作用的结果,各特征峰所代表的化学成分对其抗炎药效贡献的大小顺序为9号峰>1号峰>2号峰>6号峰>15号峰>11号峰>12号峰>7号峰>10号峰>5号峰>14号峰>3号峰>18号峰>4号峰>13号峰>16号峰>8号峰,对抗炎作用药效贡献大的前4个峰分别为9、1、2、6号峰,经过与对照品比对及质谱分析鉴定出4个峰对应的成分分别为丹参酮IIA、丹参素、丹酚酸B和3′-甲基丹酚酸B。结论丹参抗炎作用的药效是多种成分共同作用的结果,明确了丹参中丹参酮IIA、丹参素、丹酚酸B和3′-甲基丹酚酸B这4个成分对其抗炎作用的药效贡献最大,为今后探索丹参药材抗炎作用的药效物质基础提供了一种新思路。
OBJECTIVE To study the relationship between the spectrum of UPLC and anti-inflammatory effect of different extracts of Radix Salviae Miltiorrhizae, and provide the basis for clarifying the substance base of anti-inflammatory effect of Radix Salviae Miltiorrhizae. Methods The different extraction sites (S1 ~ S9) of Salvia miltiorrhiza were analyzed by UPLC method. The anti-inflammatory effects of different extraction sites were investigated by human bronchial epithelial cell inflammation model. The relationship between the spectrum and the effect was established by gray relational analysis. Results The ethanol extract of Salvia miltiorrhiza (S3), 80% ethanol eluent (S4), filtration sediment (S5) and extract layer (S9) Salvia ethanol extract of 50% ethanol (S3) the strongest ability. The efficacy of Salvia miltiorrhiza in anti-inflammatory effect is the result of the interaction of various components. The order of contribution of the chemical components represented by each characteristic peak to its anti-inflammatory efficacy is the order of peak 9> peak 1> peak 2> 6 Peak> Peak 15> Peak 11> Peak 12> Peak 7> Peak 10> Peak 5> Peak 14> Peak 3> Peak 18> Peak 4> Peak 13> Peak 16> On the 8th peak, the first 4 peaks that contributed greatly to the anti-inflammatory effects were 9, 1, 2, and 6 peaks respectively. The components corresponding to the 4 peaks identified by the comparison with the reference substance and mass spectrometry were tanshinone IIA , Danshensu, salvianolic acid B and 3’-methyl salvianolic acid B. Conclusion The anti-inflammatory effect of Salvia miltiorrhiza is the result of the interaction of various components, and the anti-inflammatory effects of the four components of Salvia Miltiorrhiza IIA, Danshensu, salvianolic acid B and 3’-methyl salvianolic acid B The largest contribution to the efficacy for the future to explore the anti-inflammatory effect of Radix Salviae Miltiorrhizae medicinal material basis provides a new idea.