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目的:本研究用PCR法检测了炎症性肠病患者粪便巨细胞病毒(cytomegalovirus,CMV)DNA含量,并与血CMV DNA等检测方法进行比较,以期揭示粪便CMV DNA含量的临床意义.方法:总结2013-01-01/2015-03-31所有在北京大学第三医院住院的炎症性肠病患者资料,收集同时检测了血清学和粪便CMV DNA含量的患者资料并进行比较.结果:共有116例炎症性肠病住院患者同时检测了血清、粪便CMV DNA含量,其中血清CMV DNA含量阳性者4例(3.4%)(血清阳性组),粪便阳性组16例(13.8%)(单纯粪便阳性组).血清阳性组粪便CMV DNA含量均为阳性,平均值为1.23×105copies(6.05×104-2.58×105copies).单纯粪便阳性组粪便CMV DNA含量平均为5.66×104copies(2.59×103-2.82×105copies).结论:对于需要明确是否存在肠道CMV感染的炎症性肠病患者,无创的粪便CMV DNA含量检测比血清学检测更为敏感.
Objective: In this study, DNA content of cytomegalovirus (CMV) in patients with inflammatory bowel disease was detected by PCR and compared with detection methods of blood CMV DNA in order to reveal the clinical significance of CMV DNA content in stool.Methods: 2013-01-01 / 2015-03-31 All patients with inflammatory bowel disease who were hospitalized in Peking University Third Hospital were collected and compared with the data of serological and stool CMV DNA content.Results: A total of 116 cases Serum and fecal CMV DNA levels were also detected in hospitalized patients with inflammatory bowel disease. Serum CMV DNA content was positive in 4 cases (3.4%) (seropositive group) and stool positive group (13.8%) (stool positive group) .The positive CMV DNA in the feces of the seropositive group was 1.23 × 105 copies (6.05 × 104-2.58 × 105 copies) .The average CMV DNA content in fecal positive group was 5.66 × 104 copies (2.59 × 103-2.82 × 105 copies) .Conclusion: Noninvasive detection of CMV DNA in feces is more sensitive than serological testing in patients with inflammatory bowel disease who need to identify the presence of intestinal CMV infection.