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采用放射免疫法测定２６例胃癌病人血清、尿液ＥＧＦ水平，同时采用免疫组化ＳＡＢＣ法检测胃粘膜组织切片中ＥＧＦＲ染色的阳性率，并与正常对照进行比较分析。结果表明：胃癌患者血清和尿ＥＧＦ水平均显著高于正常对照（３．７２±１．８３μｇ／Ｌ与１．７７±０．６０μｇ／Ｌ，Ｐ＜０．０１；１８．４４±１７．８８ｎｇ／ｍｇ与５．１９±６．３７ｎｇ／ｍｇ，Ｐ＜０．０１），胃粘膜组织切片ＥＧＦＲ染色阳性率也明显高于正常对照（７３％与１１％，Ｐ＜０．０１）。胃癌病人中，ＥＧＦＲ染色阳性者血清ＥＧＦ水平显著高于ＥＧＦＲ阴性者（Ｐ＜０．０５），但两者尿ＥＧＦ水平无显著性差异。胃癌患者血清、尿ＥＧＦ水平与胃癌大小、分化程度及淋巴结转移无显著性相关。
The serum and urinary EGF levels in 26 patients with gastric cancer were measured by radioimmunoassay. The positive rate of EGFR staining in gastric mucosa tissue sections was detected by immunohistochemical SABC method and compared with normal controls. The results showed that the serum and urinary EGF levels were significantly higher in gastric cancer patients than in normal controls (3.72±1.83 μg/L and 1.77±0.60 μg/L, P<0.01; 18.44±17.88 ng /mg and 5.19±6.37 ng/mg, P<0.01). The positive rate of EGFR staining in gastric mucosa was also significantly higher than that in normal controls (73% and 11%, P<0.01). In patients with gastric cancer, serum EGF levels were significantly higher in patients with positive EGFR staining than in those with negative EGFR (P<0.05), but there was no significant difference in urinary EGF levels between the two groups. The serum and urinary EGF levels in gastric cancer patients were not significantly related to the size, differentiation and lymph node metastasis of gastric cancer.