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目的:探讨snail、E-cadherin mRNA在宫颈癌变过程中的表达及其与临床病理特征的关系。方法:应用原位分子杂交方法,检测snail、E-cadherin mRNA在70例宫颈鳞癌、35例宫颈鳞状上皮内瘤变(CIN)和15例宫颈慢性炎症组织中的表达。结果:①70例宫颈鳞状细胞癌组织中,snail mRNA阳性率为80%(56/70),分别高于宫颈鳞状上皮内瘤变组织60%(21/35)和宫颈慢性炎症20%(3/15),差异均有统计学意义(χ2=4.773,P<0.05和χ2=18.215,P<0.05);70例宫颈鳞状细胞癌组织中,E-cadherin mRNA阳性率为21.4%(15/70),分别低于宫颈鳞状上皮内瘤变组织48.6%(17/35)和宫颈慢性炎症93.3%(14/15),差异均有统计学意义(χ2=8.113,P<0.05和χ2=28.414,P<0.05)。②snail mRNA的表达在淋巴结转移组明显高于无转移组(χ2=4.386,P<0.05),而在不同年龄、FIGO分期、病理分级及间质浸润深度之间snail mRNA水平无明显变化(P>0.05);E-cadherin mRNA的表达在淋巴结转移组明显低于无转移组(χ2=3.956,P<0.05),而在不同年龄、FIGO分期、病理分级及浸润深度之间E-cadherin mRNA水平无明显变化(P>0.05)。③宫颈组织中snail mRNA与E-cadherinmRNA的表达成负相关(P<0.05)。结论:snail mRNA高表达与E-cadherin mRNA低表达可能是宫颈癌变过程中的重要生物学标志,联合检测snail mRNA与E-cadherin mRNA有助于宫颈癌早期诊断和预后评价。
Objective: To investigate the expression of snail and E-cadherin mRNA in cervical carcinogenesis and its relationship with clinicopathological features. Methods: The expressions of snail and E-cadherin mRNA in 70 cases of cervical squamous cell carcinoma, 35 cases of cervical squamous intraepithelial neoplasia (CIN) and 15 cases of chronic cervical inflammation were detected by in situ hybridization. Results: ① The positive rate of snail mRNA in 70 cases of cervical squamous cell carcinoma was 80% (56/70), which were higher than 60% (21/35) in cervical squamous intraepithelial neoplasia and 20% in chronic cervicitis 3/15) (P <0.05). The positive rates of E-cadherin mRNA in 70 cases of cervical squamous cell carcinoma were 21.4% (χ2 = 4.773, P <0.05 and χ2 = 18.215, / 70), which were lower than those in cervical squamous intraepithelial neoplasia (48.6%, 17/35) and chronic cervicitis (93.3%, 14/15 respectively) (χ2 = 8.113, P <0.05 and χ2 = 28.414, P <0.05). ②ssnail mRNA expression was significantly higher in lymph node metastasis group than in non-metastasis group (χ2 = 4.386, P <0.05), while there was no significant difference in snail mRNA level between different stages of FIGO stage, pathological grade and depth of invasion (P> 0.05). The expression of E-cadherin mRNA in lymph node metastasis group was significantly lower than that in non-metastasis group (χ2 = 3.956, P <0.05), but there was no difference in E-cadherin mRNA expression between different age, FIGO stage, pathological grade and depth of invasion Significant changes (P> 0.05). ③ The expression of snail mRNA and E-cadherin mRNA in cervical tissue was negatively correlated (P <0.05). CONCLUSION: High expression of snail mRNA and low expression of E-cadherin mRNA may be important biological markers in carcinogenesis of cervical cancer. Combined detection of snail mRNA and E-cadherin mRNA may contribute to the early diagnosis and prognosis of cervical cancer.