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烟草２４ｋＤＰＲ蛋白裂解晚疫病菌（Ｐｈｙｔｏｐｈｔｈｏｒａｉｎｆｅｓｔａｎｓ）孢子囊并抑制其菌丝生长，是马铃薯抗晚疫病遗传工程所需要的核心元件。我们以烟草（ＮｉｃｏｔｉａｎａｔａｂａｃｕｍＸａｎｘｉ－ｎｃＮＮ）基因组ＤＮＡ为模板，通过合成５′－端及３′－端的一对特异引物，利用多聚酶链式反应（ＰＣＲ）扩增得到了缺失３′－端ＣＴＰＰ的２４ｋＤ蛋白基因，并克隆到Ｅ．ｃｏｌｉ质粒上。序列分析表明，我们得到的２４ｋＤ蛋白基因由６９６个核苷酸组成，编吗２３２个氨基酸残基组成的多肽，包括５′－端２５个氨基酸残基的信号肽，与国外报道的ＡＰ２４蛋白基因序列一致。
The tobacco 24kDPR protein cleaves the sporangia of Phytophthora infestans and inhibits its mycelial growth, and is the core element required for the genetic engineering of late blight resistance in potato. Using Nicotiana tabacum Xanxi-ncNN genomic DNA as a template, a 24 kD protein lacking 3’-terminal CTPP was amplified by polymerase chain reaction (PCR) amplification by synthesizing a pair of specific primers at 5’-end and 3’-end Gene, and cloned into E. coli plasmid. Sequence analysis showed that the 24kD protein we obtained consisted of 696 nucleotides and encoded a polypeptide of 232 amino acid residues, including the signal peptide of 25 amino acid residues at the 5’-end. Compared with the reported AP24 protein gene The same sequence.