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目的探讨不同人群血清对麻疹流行株与疫苗株中和能力的差异。方法采集麻疹患者急性期与恢复期血清、麻疹疫苗免疫前后儿童血清以及流动人口血清,分别对疫苗株沪191与2005年麻疹流行株进行中和抗体(NT)滴度测定。同时,利用疫苗株、浙江省当地流行株制备动物免疫血清,与相应毒株进行交叉中和试验,测定各毒株之间抗原比。结果疫苗免疫后儿童血清对疫苗株的中和抗体几何平均滴度(GMT)为50.82,高于流行株MVi/ZJ/05/7(GMT值为27.35)1.86倍;患者恢复期血清对流行株的中和能力(GMT值为386.95)显著高于疫苗株(GMT值为151.83);流动人口血清对流行株中和抗体GMT值均小于疫苗株,差异为2.22～4.17倍;MVi/zJ/99/1、MVi/ZJ/04/1和MVi/ZJ/05/7与疫苗株间抗原比分别为4.28、5.24和5.66。此外,部分患者急性期血清对疫苗株存在低滴度的中和抗体,GMT值1:4左右。结论不同人群血清对麻疹疫苗株与流行株的中和能力的差异有统计学意义,低滴度的疫苗抗体不能有效保护个体免受麻疹流行毒株的侵袭,应加强麻疹病毒变异与疫苗效果的相关研究。
Objective To investigate the difference of neutralization ability between different populations of measles epidemic strains and vaccine strains. Methods Serum of measles patients during the acute phase and convalescent phase, serum of measles vaccine before and after immunization and serum of floating population were collected. The neutralizing antibody (NT) titers of measles epidemic strains were determined respectively. At the same time, using the vaccine strain, Zhejiang Province, the local epidemic strains prepared animal immune sera, and the corresponding strains were cross-neutralization test to determine the antigen ratio between the strains. Results The geometric mean titer (GMT) of the neutralizing antibody to the vaccine strain after vaccination was 50.82, which was 1.86 times higher than that of the MVi / ZJ / 05/7 strain (27.35 GMT) (GMT = 386.95) was significantly higher than that of the vaccine strain (GMT = 151.83). The neutralizing antibodies (GMTs) of the circulating strains were less than that of the vaccine strains, with a difference of 2.22-4.17 times; MVi / zJ / 99 / 1, MVi / ZJ / 04/1 and MVi / ZJ / 05/7 and vaccine strains were 4.28,5.24 and 5.66 respectively. In addition, some patients with acute phase serum vaccine strains have low titers of neutralizing antibodies, GMT value of about 1: 4. Conclusions The differences of neutralizing abilities of measles vaccine strains and epidemic strains in different populations are statistically significant. Low-titer vaccine antibodies can not effectively protect individuals from epidemic strains of measles, and measles virus mutation and vaccine efficacy should be strengthened Related research.