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通过挖掘控制重要农艺性状的基因以提高农作物产量是育种学家一直关注的问题。然而由于农作物遗传背景复杂且基因组庞大,该过程往往步骤繁琐,耗时长且成本较高。Mapping-by-sequencing(利用高通量测序进行基因定位,MBS)利用二代测序技术和遗传分离群体混池建库分析的方法成功解决了上述问题,加快了农作物重要农艺性状的基因定位同时也降低了研究成本。该方法已经成功应用于重要农艺性状相关联的突变位点鉴别和基因定位。本文综述了Mapping-by-sequencing的实验原理,并总结了该方法在水稻、大麦、小麦及玉米等农作物中与目标农艺性状相关基因定位的应用。
It has been a constant concern for breeders by digging genes that control important agronomic traits to increase crop yields. However, due to the complex genetic backgrounds and large genomes of crops, the process is often cumbersome, time-consuming and costly. Mapping-by-sequencing (MBS) uses the second-generation sequencing technology and the genetic analysis of hybrid pool pool construction to successfully solve the above problems and accelerate the gene mapping of important agronomic traits in crops. Reduce the cost of research. This method has been successfully applied to the identification of mutational sites and gene mapping related to important agronomic traits. This paper reviews the experimental principle of Mapping-by-sequencing and summarizes the application of this method in the mapping of genes related to the target agronomic traits in crops such as rice, barley, wheat and maize.