目的观察颐和春口服液对肾阳虚大鼠肾功能的保护作用,并初步探讨其机制。方法用腺嘌呤灌胃制备肾阳虚大鼠模型。实验分为正常对照组、肾阳虚模型组、肾阳虚阳性药物治疗组和肾阳虚颐和春治疗组。给药10d后观察颐和春口服液对肾阳虚大鼠体重、肾指数、肾功能及肾形态学改变,并从凋亡和抗氧化方面探讨其保护机制。HE染色观察肾形态学改变、生化法测定血清谷胱甘肽过氧化物酶(GSH-PX)、血尿素氮(BUN)和肌酐(Cr),末端标记法测定肾组织凋亡。结果颐和春可改善肾阳虚引起的大鼠体重下降,使肾指数增加,降低血清肌酐(Cr)和尿素氮(BUN)水平,改善肾形态学表现,减轻肾小管上皮细胞凋亡,提高血清谷胱甘肽过氧化酶(GSH-PX)活性。结论颐和春口服液对肾阳虚大鼠肾功能有明显的保护作用,可能与提高抗凋亡和抗氧化能力有关。 Objective To observe the protective effect of Yihechun Oral Liquid on renal function in rats with kidney-yang deficiency and to explore its mechanism. Methods Adenine was used to prepare rat model of deficiency of kidney yang. The experiment was divided into normal control group, kidney yang deficiency model group, yang deficiency group and yang-yang-yi-chun treatment group. After administration for 10 days, the body weight, renal index, renal function and kidney morphology of Yiyangchun Oral Liquid were observed. The mechanism of protection was also discussed from the aspects of apoptosis and antioxidation. The changes of renal morphology were observed by HE staining. The levels of serum glutathione peroxidase (GSH-PX), blood urea nitrogen (BUN) and creatinine (Cr) were measured by biochemical methods. Results Yihechun can reduce the weight loss of rats induced by kidney-yang deficiency, increase the index of kidney, lower the levels of serum creatinine (Cr) and blood urea nitrogen (BUN), improve the renal morphology, reduce the apoptosis of renal tubular epithelial cells and increase Serum glutathione peroxidase (GSH-PX) activity. Conclusion Yihechun oral liquid has obvious protective effect on renal function in rats with kidney-yang deficiency, which may be related to the improvement of anti-apoptosis and anti-oxidation ability.