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目的:研究应变量不同的两种机械力对PFD与非PFD患者的骶韧带成纤维细胞细胞骨架形态影响及骨架相关蛋白vimentin与α-actintin表达的调节。方法:以12例POP患者的骶韧带为病例组,以同期(非POP)因子宫良性病变行子宫全切的15例患者为对照组,对骶韧带成纤维细胞原代培养后进行形态学观察和细胞鉴定。采用Flexcercell4000柔性基底拉伸加载系统对实验对象进行加载,观察不同机械力对两组成纤维细胞骨架中vimentin的影响,同时考察受力前后骨架相关蛋白α-actintin表达含量的变化。结果:在机械力作用下骶韧带成纤维细胞的形态及排列方式变化,随着受力时间的延长形态由原来的多形性成为单一的长梭形,排列方向趋于规则并垂直于最大应变方向,以24 h最明显。结论:机械力在PFD盆底组织细胞骨架的变化中发挥着重要作用,机械力在PFD的发病机制中有一定的作用。
OBJECTIVE: To study the effect of two mechanical forces with different amounts of strain on cytoskeleton morphology of sacral ligament fibroblasts and the regulation of the expression of vimentin and α-actintin in PFD and non-PFD patients. Methods: The sacral ligaments of 12 patients with POP were selected as the case group and 15 patients underwent uterine resection in the same period (non-POP) due to uterine benign disease. The morphological changes of the sacral ligament fibroblasts were observed after primary culture. And cell identification. Flexcercell4000 flexible substrate tensile loading system was used to load the experimental object, to observe the different mechanical forces on the two groups of fibroblast cytoskeleton vimentin influence, at the same time to examine stress changes before and after the matrix-related protein expression of α-actintin. Results: The morphology and arrangement of sacral ligament fibroblasts under mechanical force changed from original pleomorphism to single fusiform shape with the extension of stress time, and the arrangement direction tended to be regular and perpendicular to the maximum strain Direction, the most obvious 24 h. CONCLUSION: Mechanical force plays an important role in the change of cytoskeleton in PFD pelvic floor. The mechanical force has a certain role in the pathogenesis of PFD.