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目的探讨戊四氮诱导发育期大鼠癫癎持续状态(SE)后对海马内齿状回颗粒细胞神经发生的影响以及N-甲基D-天冬氨酸受体(NMDAR)拮抗剂MK-801对此结果的抑制作用,从而研究癫癎发作后发育脑海马内神经发生及NMDAR在神经发生中的作用。方法SD大鼠7,14,21,28d4个日龄组共216只,每组均为54只,每个日龄组大鼠随机分SE组、MK-801组和正常对照组,每组18只。采用5-溴脱氧尿核苷(BrdU)标记新生细胞,再以β微管蛋白Ⅲ(TuJ1)、胶质原纤维酸性蛋白(GFAP)分别标记早期神经元和胶质细胞的单、双标免疫组织化学方法,检测PTZ诱导癫癎持续状态后发育鼠海马齿状回(dentategyrus,DG)神经发生,并用MK-801治疗后观察对其的影响。结果BrdU注射后第7天和第14天,SE组各日龄幼鼠齿状回BrdU阳性细胞数明显高于同日龄的正常对照组,其中约有80%同时表达TuJ1;MK-801组BrdU阳性细胞数较SE组明显减少(P<0.01),而在第28天三组之间BrdU阳性细胞数无明显差异(P>0.05)。结论癫癎发作可增加幼鼠齿状回颗粒细胞的神经发生,而NMDAR在癫癎后的神经发生中起着促进作用。
Objective To investigate the effects of epileptic seizure (SE) on the neurogenesis of hippocampal gyrus of granulosa cells and the effects of N-methyl D-aspartate receptor (NMDAR) antagonist MK- 801 on the inhibition of this result, to study the development of epileptic hippocampal neurogenesis and NMDAR role in neurogenesis. METHODS: A total of 216 Sprague-Dawley (SD) rats aged 4 days, 7 days, 14 days, 21 days, 28 days were divided into SE group, MK-801 group and normal control group only. BrdU-labeled neonatal cells were used to label single and double-stranded immunocytes of early neurons and glial cells with TuJ1 and GFAP Histochemistry was used to detect the neurogenesis of dendritic dentate gyrus (DG) in PTZ-induced status epilepticus. The effects of MK-801 on the neurogenesis were observed. Results On the 7th day and the 14th day after BrdU injection, the number of BrdU-positive cells in the dentate gyrus of SE group was significantly higher than that of the normal control group at the same age, of which about 80% of the BrdU positive cells expressed TuJ1; BrdU Compared with SE group, the number of positive cells decreased significantly (P <0.01), while there was no significant difference in the number of BrdU positive cells between the three groups on the 28th day (P> 0.05). Conclusion Epileptic seizures can increase the neurogenesis of dentate gyrus granulosa cells in young rats, while NMDAR plays a role in the neurogenesis after epileptic seizures.