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目的：研究急性坏死性胰腺炎（ＡＮＰ）时肠道细菌易位情况，探讨肠道是否为继发性胰腺感染的细菌来源。方法：采用ＰＵＣ１８质粒载体大肠肝菌（ＪＭ１０９）示踪法，将质粒菌ＪＭ１０９定植于１５只犬肠道后，随机分为ＡＮＰ组（８只）及对照组（７只），前者复制ＡＮＰ模型。每日进行血培养，第７日活杀后作脏器细菌培养。对耐氨苄青霉素菌株进行酶切分析及琼脂糖凝胶电泳。结果：胰腺炎犬除肾及脾脏外，其余脏器均发现有ＪＭ１０９菌易位，以胰腺易位率最高（７５％，６／８）；发病后２４小时血培养中找到质粒菌ＪＭ１０９阳性率高达５０％。结论：ＡＮＰ发病后２４小时即可出现细菌易位，肠道细菌易位是继发性胰腺感染的可能机制
Objective: To study the intestinal bacterial translocation during acute necrotizing pancreatitis (ANP) and to investigate whether the intestinal tract is the source of bacteria for secondary pancreatic infection. Methods: The colon bacillus of E. coli JM109 was colonized in 15 dogs by PUC18 plasmid vector E.coli JM109 and divided into ANP group (n = 8) and control group (n = 7) randomly. The former replicated ANP model . Daily blood culture, the seventh day after killing live organ culture. Resistant ampicillin strains were digested and analyzed by agarose gel electrophoresis. Results: In addition to the kidney and spleen, dogs in the other organs were found to have the translocation of JM109, with the highest translocation rate (75%, 6/8). The positive rate of plasmid-producing bacteria JM109 was found in the blood culture at 24 hours after onset Up to 50%. CONCLUSIONS: Bacterial translocation occurs 24 hours after the onset of ANP. Intestinal bacterial translocation is a possible mechanism of secondary pancreatic infection