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为了研究大苞萱草植株无菌再生能力,从而摸索植物组织生长各阶段所需的不同激素种类及其适宜浓度,为大规模扩繁、培育转基因新品种提供参考数据奠定基础,试验采用组织培养技术及响应面法分析大苞萱草植物组织培养最佳条件。结果表明:大苞萱草植物组织培养最佳的外植体消毒方案为75%酒精30 s+0.1%升汞7 min;最佳愈伤诱导培养基为MS+6-BA 2 mg/L+萘乙酸(NAA)0.3 mg/L+2,4-D 0.3 mg/L;最佳生根培养基为1/2MS+NAA 0.3 mg/L+2 g/L活性炭。
In order to study the aseptic regenerative ability of Hemerocallis fulva, to explore different kinds of hormones needed by different stages of plant tissue growth and its appropriate concentration, which laid the foundation for large-scale propagation and breeding of new transgenic varieties. Tissue culture technology Response surface methodology was used to analyze the optimal conditions for tissue culture of Hemerocallus. The results showed that the optimal explants for the tissue culture of Heberry cameaural was 75% alcohol 30 s + 0.1% mercuric chloride for 7 min. The best callus induction medium was MS + 6-BA 2 mg / L + NAA (NAA) 0.3 mg / L and 2,4-D 0.3 mg / L. The best rooting medium was 1 / 2MS + NAA 0.3 mg / L + 2 g / L activated carbon.