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目的研究某市典型环境水有机提取物的细胞毒性及致突变性。方法采集某市饮用水源水、城市回用水、河水及公园景观水水样,以固相萃取技术提取水中有机物,以MTT法检测有机提取物对中国仓鼠肺细胞(CHL细胞)的细胞毒性,以Ames试验检测有机提取物的致突变性。结果水源水、城市回用水、河水、景观水有机提取物引起CHL细胞毒性的IC50分别为550、90、250、500 ml/ml。Ames试验显示,在无代谢活化系统下,城市回用水在500 ml/皿及以上、河水在1 000ml/皿及以上TA98和TA100两菌株均呈阳性;景观水在2 000 ml/皿TA98呈阳性;在有代谢活化系统下,城市回用水TA98菌株在500 ml/皿及以上、TA100在1 000 ml/皿及以上,河水在1 000 ml/皿及以上两菌株均呈阳性,水源水及景观水未见致突变性。结论城市典型环境水有机提取物在一定剂量下,对CHL细胞均产生毒性作用。在无代谢活化系统下,城市回用水、河水及公园景观水有机提取物具有致突变作用。在有代谢活化系统下,城市回用水、河水有机提取物具有致突变作用。
Objective To study the cytotoxicity and mutagenicity of organic extracts of typical environment in a certain city. Methods Water samples from drinking water sources, urban water reuse, river water and landscape were collected from a city. The organic compounds in water were extracted by solid phase extraction. The cytotoxicity of organic extracts on Chinese hamster lung cells (CHL cells) Ames test was used to test the mutagenicity of organic extracts. Results The IC50 of CHL cytotoxicity caused by water source, urban water reuse, river water and landscape water organic extract were 550, 90, 250 and 500 ml / ml, respectively. Ames test showed that in the absence of metabolic activation system, the urban reuse water at 500 ml / dish and above, the water at 1000ml / dish and above TA98 and TA100 two strains were positive; landscape water at 2000ml / dish TA98 positive ; Under the metabolic activation system, the urban reuse water TA98 strains at 500 ml / dish and above, TA100 at 1000 ml / dish and above, the river water at 1000 ml / dish and above two strains were positive, water source and landscape No mutagenicity in water. Conclusions The organic extracts from urban typical environment have toxic effects on CHL cells under certain dose. In the absence of metabolic activation system, urban water reuse, river water and park landscape water organic extract has a mutagenic effect. In a metabolic activation system, the city back to water, river organic extract has a mutagenic effect.